School of BioSciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, UK.
Veterinary Laboratories Agency, Woodham Lane, Addlestone, UK.
J Med Microbiol. 2011 May;60(Pt 5):661-669. doi: 10.1099/jmm.0.026658-0. Epub 2011 Jan 13.
Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.
家蚕幼虫已被证明易感染空肠弯曲菌感染，我们的研究描述了这种感染模型。在感染空肠弯曲菌人类分离株后，可见细菌存在于受挑战幼虫的血腔和肠道中，并且肠道广泛受损。细菌存在于血腔的细胞外和细胞相关部分，并且表明空肠弯曲菌可以在昆虫细胞中存活。最后，我们使用该模型进一步筛选了属于不同 MLST 型的 67 个空肠弯曲菌分离株。属于 ST257 的分离株在 Galleria 模型中最具毒力，而属于 ST21 的分离株毒力最低。
