Gorskaya U F, Danilova T A, Mesentzeva M V, Shapoval I M, Narovlyansky A N, Nesterenko V G
Laboratory for Regulation of Immunity, Laboratory for Microbiology of Latent Infections, Laboratory of Cytokines, N F Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2010 Oct;149(4):425-7. doi: 10.1007/s10517-010-0961-9.
Administration of S. typhimurium microbial mass to mice was followed by a significant increase (by 3-4 times) in the efficiency of cloning and number of stromal precursors in the femoral bone marrow. These parameters were maximum on days 1-3, but returned to normal by the 8th-15th day after immunization. As differentiated from intact animals, the expression of genes for proinflammatory cytokines IL-1β (day 1 after immunization), IL-6 (days 1-3), TNF-α (days 1, 3, and 6), and IFN-α (days 1-3) was detected in bone marrow cultures from immunized mice. The expression of genes for IFN-γ, IL-18, and IFN-α was decreased on days 1, 3, and 6 after immunization of animals, respectively. Gene expression for the anti-inflammatory cytokine IL-4 was observed on day 6 after immunization. Therefore, this system was not characterized by a decrease in the immune response of stromal cells. The stromal component of hemopoietic and lymphoid organs has the vector of influences in response to bacterial antigens. This vector is directed to the stimulation and progression, but not to the suppression of immune reactions. Our results indicate that resident stromal cells play a role in the immune response of the body.
给小鼠施用鼠伤寒沙门氏菌菌体后，股骨骨髓中的克隆效率和基质前体细胞数量显著增加（增加3至4倍）。这些参数在免疫后第1至3天达到最大值，但在第8至15天恢复正常。与未免疫动物不同，在免疫小鼠的骨髓培养物中检测到促炎细胞因子IL-1β（免疫后第1天）、IL-6（第1至3天）、TNF-α（第1、3和6天）和IFN-α（第1至3天）的基因表达。在动物免疫后第1、3和6天，IFN-γ、IL-18和IFN-α的基因表达分别降低。在免疫后第6天观察到抗炎细胞因子IL-4的基因表达。因此，该系统的特征不是基质细胞免疫反应降低。造血和淋巴器官的基质成分在对细菌抗原的反应中具有影响向量。该向量旨在刺激和促进免疫反应，而不是抑制免疫反应。我们的结果表明，驻留基质细胞在机体的免疫反应中发挥作用。
