Gorskaya Yu F, Danilova T A, Lebedinskaya O V, Lunin V G, Grabko V I, Sharapova N E, Nesterenko V G
Laboratory of Immunity Regulation, N. F. Gamaleya Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2011 Sep;151(5):607-11. doi: 10.1007/s10517-011-1394-9.
Immunization of CBA mice with killed group A streptococcus (type 5) vaccine changed the counts of stromal precursor cells (CFC-F) in bone marrow transplants at different donor-recipient combinations (normal, N, or immune, I). CFC-F counts in bone marrow transplants from normal mice transplanted to immunized animals decreased 4-6-fold depending on the transplant age in comparison with similar transplants in normal recipients. The percentage of CFC-F colonies with alkaline phosphatase (osteogenesis marker) activity decreased more than 2-fold. Similarly, the count of CFC-F in the transplants was 2-fold lower during delayed (7 months) period after bone marrow transplantation from immunized donors (8-12 days after the end of immunization) to intact recipients, while 2 months after transplantation it was 3-fold lower. The mean optical density of the bone capsule in preparations stained for glycogen and alkaline phosphatase was 1.5-3 times lower in the N-->I and I-->N experiments in comparison with the control (N-->N). On the other hand, CFC-F count in the femoral bone marrow of immunized animals was significantly (3.5-2.5 times) higher during the period from 8 days to 8 months after the end of immunization compared to CFC-F count in the femoral bone marrow of intact mice. These results attest to a significant prolonged effect of streptococcal antigens on the bone marrow stromal tissue. These data also indicate that not all CFC-F, the counts of which increased in response to antigens, are responsible for transplantability of the stromal tissue in heterotopic transplantation. Immunization by streptococcal antigens seemed to suppress transplantability and osteogenic activity of stromal stem cells. The efficiency of CFC-F cloning in mouse bone marrow cultures increased significantly (2-3-fold) in the presence of sera from immune mice. The levels of TNF-α and IFN-γ were low in this serum (2.7 and 6 times lower, respectively) in comparison with normal serum. Presumably, the effects of streptococcal antigens on stromal tissue were mediated through serum cytokines.
用灭活的A组5型链球菌疫苗免疫CBA小鼠，会改变不同供体-受体组合（正常，N，或免疫，I）的骨髓移植中基质前体细胞（CFC-F）的数量。与正常受体中的类似移植相比，从正常小鼠移植到免疫动物的骨髓移植中的CFC-F数量根据移植年龄减少了4至6倍。具有碱性磷酸酶（成骨标记物）活性的CFC-F集落百分比下降了2倍以上。同样，在从免疫供体（免疫结束后8 - 12天）向未免疫受体进行骨髓移植后的延迟期（7个月），移植中的CFC-F数量降低了2倍，而移植后2个月则降低了3倍。在糖原和碱性磷酸酶染色的制剂中，与对照（N→N）相比，在N→I和I→N实验中骨囊的平均光密度降低了1.5至3倍。另一方面，与未免疫小鼠股骨骨髓中的CFC-F数量相比，免疫动物在免疫结束后8天至8个月期间股骨骨髓中的CFC-F数量显著更高（3.5至2.5倍）。这些结果证明链球菌抗原对骨髓基质组织有显著的长期影响。这些数据还表明，并非所有因抗原而数量增加的CFC-F都对异位移植中基质组织的可移植性负责。链球菌抗原免疫似乎抑制了基质干细胞的可移植性和成骨活性。在存在免疫小鼠血清的情况下，小鼠骨髓培养物中CFC-F克隆效率显著提高（2至3倍）。与正常血清相比，该血清中TNF-α和IFN-γ水平较低（分别低2.7倍和6倍）。推测链球菌抗原对基质组织的作用是通过血清细胞因子介导的。
