Tripathi R C, Tripathi B J, Park J K
Department of Ophthalmology and Visual Science, University of Chicago, IL 60637.
Exp Eye Res. 1990 Nov;51(5):545-52. doi: 10.1016/0014-4835(90)90085-9.
We investigated the presence of urokinase-type plasminogen activator (u-PA) in various structures of the human eye by using a biotin-strepavidin complex immunoperoxidase technique with a monoclonal antibody specific for human u-PA. A moderate to intense reaction product was seen in the corneal endothelium, episclera, sclera, iris muscles, lens equator and posterior capsule, peripheral vitreous, epithelium of pars plana, retinal pigment epithelium, axons, uveal fibroblasts, optic nerve fibers, and extraocular muscles. A weak reaction product was seen in the conjunctival and corneal epithelium, the trabecular meshwork, melanocytes and stroma of the iris and choroid, and the posterior layers of the retina. u-PA was not localized in the corneal stroma, conjunctival goblet cells, and certain parts of the lens. Comparison of the present results with those of our previous study (Tripathi, Geanon and Tripathi, 1987, Ophthalmology, 94, 1434-8) on the localization of tissue plasminogen activator (t-PA) in the eye indicate that the distribution of u-PA and t-PA overlaps in many tissues, but differs significantly in others, and that this finding may be related to their complementary and specific roles. As in other body systems, u-PA in ocular tissues is probably involved in processes such as fibrinolysis, wound healing, turnover of intra- and extracellular macromolecules, muscles and neuromuscular regeneration, synapse formation, and maintenance of general neuronal function.
我们采用生物素 - 链霉亲和素复合物免疫过氧化物酶技术，使用针对人尿激酶型纤溶酶原激活剂（u - PA）的单克隆抗体，研究了人眼各种结构中u - PA的存在情况。在角膜内皮、巩膜上层、巩膜、虹膜肌肉、晶状体赤道和后囊、周边玻璃体、睫状体扁平部上皮、视网膜色素上皮、轴突、葡萄膜成纤维细胞、视神经纤维和眼外肌中可见中度至强烈的反应产物。在结膜和角膜上皮、小梁网、虹膜和脉络膜的黑素细胞及基质以及视网膜后层可见微弱的反应产物。u - PA未定位在角膜基质、结膜杯状细胞和晶状体的某些部位。将本研究结果与我们之前关于组织纤溶酶原激活剂（t - PA）在眼内定位的研究（Tripathi、Geanon和Tripathi，1987年，《眼科学》，94卷，1434 - 1438页）结果进行比较表明，u - PA和t - PA在许多组织中的分布重叠，但在其他组织中差异显著，这一发现可能与其互补和特定作用有关。与其他身体系统一样，眼组织中的u - PA可能参与诸如纤维蛋白溶解、伤口愈合、细胞内外大分子周转、肌肉和神经肌肉再生、突触形成以及一般神经元功能维持等过程。
