Department of Gene Regulation, HZI, Build. D, Inhoffenstr. 7, 38124, Braunschweig, Germany.
Angiogenesis. 2011 May;14(2):163-72. doi: 10.1007/s10456-011-9200-x. Epub 2011 Jan 15.
Recently, we isolated and characterized resident endothelial progenitor cells from the lungs of adult mice. These cells have a high proliferation potential, are not transformed and can differentiate into blood- and lymph-vascular endothelial cells under in vitro and in vivo conditions. Here we studied the secretome of these cells by nanoflow liquid chromatographic mass spectrometry (LC-MS). For analysis, 3-day conditioned serum-free media were used. We found 133 proteins belonging to the categories of membrane-bound or secreted proteins. Thereby, several of the membrane-bound proteins also existed as released variants. Thirty-five proteins from this group are well known as endothelial cell- or angiogenesis-related proteins. The MS analysis of the secretome was supplemented and confirmed by fluorescence activated cell sorting analyses, ELISA measurements and immunocytological studies of selected proteins. The secretome data presented in this study provides a platform for the in-depth analysis of endothelial progenitor cells and characterizes potential cellular markers and signaling components in hem- and lymphangiogenesis.
最近，我们从成年老鼠的肺部中分离并鉴定了常驻的内皮祖细胞。这些细胞具有高增殖潜能，不会转化，并且可以在体外和体内条件下分化为血液和淋巴管内皮细胞。在这里，我们通过纳米流液相色谱-质谱联用（LC-MS）研究了这些细胞的分泌组。为此，使用了 3 天的无血清条件培养基进行分析。我们发现了 133 种属于膜结合或分泌蛋白类别的蛋白质。因此，该组中的几种膜结合蛋白也存在作为释放变体。来自该组的 35 种蛋白质是众所周知的与内皮细胞或血管生成相关的蛋白质。通过荧光激活细胞分选分析、ELISA 测量和选定蛋白质的免疫细胞化学研究对分泌组的 MS 分析进行了补充和验证。本研究中提供的分泌组数据为内皮祖细胞的深入分析提供了一个平台，并描绘了潜在的细胞标记物和在血液和淋巴管生成中的信号成分。
