Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
Proteomics. 2011 Feb;11(4):691-708. doi: 10.1002/pmic.201000402. Epub 2011 Jan 17.
Within a mammalian organism, the interaction among cells both at short and long distances is mediated by soluble factors released by cells into the extracellular environment. The secreted proteins may involve extracellular matrix proteins, proteinases, growth factors, protein hormones, immunoregulatory cytokines, chemokines or other bioactive molecules that have a direct impact on target cell phenotype. Stem cells of mesenchymal, adipose, neural and embryonic origin, fibroblast feeder cells as well as primary isolates of astrocytes, endothelial and muscle cells have recently become targets of intensive secretome profiling with the search for proteins regulating cell survival, proliferation, differentiation or inflammatory response. Recent advances and challenges of the stem cell and primary cell secretome analysis together with the most relevant results are discussed in this review.
在哺乳动物体内，细胞之间的短距离和长距离相互作用是由细胞释放到细胞外环境中的可溶性因子介导的。分泌蛋白可能涉及细胞外基质蛋白、蛋白酶、生长因子、蛋白激素、免疫调节细胞因子、趋化因子或其他对靶细胞表型有直接影响的生物活性分子。最近，间充质、脂肪、神经和胚胎来源的干细胞、成纤维细胞饲养细胞以及星形胶质细胞、内皮细胞和肌肉细胞的原代分离物已成为密集分泌组谱分析的目标，以寻找调节细胞存活、增殖、分化或炎症反应的蛋白质。本文讨论了干细胞和原代细胞分泌组分析的最新进展和挑战以及最相关的结果。
