Owen J A, Grahl K T, Zador A, Dorer D R, Klein L, Rubin M B
Department of Biology, Haverford College, PA 19041.
Immunol Cell Biol. 1990 Aug;68 ( Pt 4):269-76. doi: 10.1038/icb.1990.37.
The molecular requirements for recognition of antigen-modified cells by cytotoxic T lymphocyte precursors (CTLp) and their activated progeny, cytotoxic T lymphocytes (CTL), have been compared using haptenated stimulator and target cells. The antigen density requirements of T cell recognition by fluorescein-specific CTLp and CTL derived both from naive mice and from animals previously primed in vivo were determined. The cell surface hapten concentration required to stimulate CTLp cannot be distinguished from that required on target cells for lysis by their mature daughter CTL 5-7 days later. However, if the CTL (and their precursor CTLp) are derived from mice primed in vivo with hapten-conjugated cells, they require lower cell surface hapten densities for recognition than do the analogous T cell populations from naive animals. Thus, the maturation of CTLp into CTL during 5-7 days in vitro does not result in any functionally relevant change in the nature or density of antigen receptors on the surface of the T cell. This is in contrast to the apparent selection which occurs over longer time periods in vivo following priming.
利用半抗原化的刺激细胞和靶细胞，比较了细胞毒性T淋巴细胞前体（CTLp）及其活化后代细胞毒性T淋巴细胞（CTL）识别抗原修饰细胞的分子要求。测定了来自未接触过抗原的小鼠以及先前在体内已接触过抗原的动物的荧光素特异性CTLp和CTL识别T细胞的抗原密度要求。刺激CTLp所需的细胞表面半抗原浓度与5-7天后其成熟子代CTL用于裂解靶细胞所需的浓度并无差异。然而，如果CTL（及其前体CTLp）来源于在体内用半抗原偶联细胞致敏的小鼠，那么与来自未接触过抗原动物的类似T细胞群体相比，它们识别所需的细胞表面半抗原密度更低。因此，体外培养5-7天期间CTLp成熟为CTL的过程不会导致T细胞表面抗原受体的性质或密度发生任何功能上相关的变化。这与体内初次致敏后较长时间段内发生的明显选择形成对比。
