Ballas Z K, Ahmann G B
Cell Immunol. 1983 Feb 15;76(1):81-93. doi: 10.1016/0008-8749(83)90350-7.
Spleen cells obtained from normal mice were cultured with interleukin 2 (IL2); no antigen was added. After 4-5 days, these cultures contained effector cells which lysed autologous spleen target cells that were modified with 2,4,6-trinitrobenzene sulfonic acid or fluorescein isothiocyanate. No killing was seen on unmodified spleen target cells. These effector cells were Thy 1+, Lyt 1-, 2+ and were derived from Thy 1+ precursor cells. IL2 preparations induced the generation of such cytotoxic T lymphocytes (CTL) in a dose-dependent manner. IL2-induced CTL were shown to be different from the natural killer (NK) cells augmented by IL2 by virtue of their time of appearance in culture, by cold-target competition, and by different cell-surface markers. These results demonstrate that the IL2 signal may be sufficient for the induction of the differentiation of CTL precursors in the absence of an antigenic signal.
从正常小鼠获取的脾细胞用白细胞介素2（IL2）进行培养，未添加抗原。4 - 5天后，这些培养物中含有效应细胞，它们能裂解经2,4,6 - 三硝基苯磺酸或异硫氰酸荧光素修饰的自体脾靶细胞。未修饰的脾靶细胞未见杀伤作用。这些效应细胞为Thy 1 +、Lyt 1 -、2 +，来源于Thy 1 +前体细胞。IL2制剂以剂量依赖方式诱导此类细胞毒性T淋巴细胞（CTL）的产生。通过培养中出现的时间、冷靶竞争以及不同的细胞表面标志物，表明IL2诱导的CTL不同于由IL2增强的自然杀伤（NK）细胞。这些结果证明，在没有抗原信号的情况下，IL2信号可能足以诱导CTL前体的分化。
