微小膜壳绦虫表面抗原的纯化及免疫反应性
Purification and immunologic reactivity of Hymenolepis diminuta surface antigens.
作者信息
Krawczuk S, Rode W, Machnicka B
机构信息
Institute of Parasitology, Polish Academy of Sciences, Warszawa, Poland.
出版信息
Parasitol Res. 1990;76(8):707-11. doi: 10.1007/BF00931091.
The outer part of Hymenolepis diminuta tegument was extracted with 3 M KCl. The antigen was adsorbed from the extract on an affinity column containing H. diminuta-infected rat immunoglobulin immobilized on Sepharose 4B and could be eluted with 2.5 M urea at pH 2.8. Analysis of polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of both the crude extract and material eluted from the column showed that the latter was markedly purified. Double diffusion and immunoelectrophoresis revealed 11 and 4 antigenic components in the crude extract and purified preparation, respectively.
微小膜壳绦虫皮层的外部用3M氯化钾提取。抗原从提取物中吸附到一个亲和柱上，该亲和柱含有固定在琼脂糖4B上的感染微小膜壳绦虫的大鼠免疫球蛋白，并且可以在pH2.8时用2.5M尿素洗脱。对粗提取物和从柱上洗脱的物质进行聚丙烯酰胺凝胶电泳（PAGE）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析表明，后者得到了显著纯化。双向扩散和免疫电泳分别显示粗提取物和纯化制剂中有11种和4种抗原成分。
Zotero 插件