Machnicka B, Krawczuk S, Walski M
Folia Histochem Cytobiol. 1986;24(1):65-70.
The glycocalyx of Hymenolepis diminuta (Cestoda, Cyclophyllidea) was isolated using 0.02 M EDTA or 3 M KCl. It was shown in the electron micrographs that 0.02 M EDTA did not damage the tapeworm plasma membrane, eliminating glycocylax only, in contrast to 3 M KCl which disrupted tegument up to the basal membrane. The protein analysis of extracts and the supernatant of homogenate of the whole tapeworm strobila by polyacrylamide gel electrophoresis (PAGE) and dodecyl sulphate-polyacrylamide gel electrophoresis (SDS) electrophoresis revealed that the substance extracted with 3 M KCl was more abundant in protein fractions than the two remaining ones. The substance extracted with 0.02 EDTA, collecting the tapeworm glycocalyx possessed the smallest amount of protein fractions, however, some of them were more abundant.
使用0.02 M乙二胺四乙酸（EDTA）或3 M氯化钾分离微小膜壳绦虫（绦虫纲，圆叶目）的糖萼。电子显微镜照片显示，0.02 M EDTA不会损伤绦虫质膜，仅去除糖萼，而3 M氯化钾会破坏皮层直至基底膜。通过聚丙烯酰胺凝胶电泳（PAGE）和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS）对整个绦虫节片匀浆的提取物和上清液进行蛋白质分析，结果表明，用3 M氯化钾提取的物质在蛋白质组分中比其余两种更丰富。用0.02 EDTA提取的物质，即收集到的绦虫糖萼，蛋白质组分含量最少，不过其中一些组分含量更高。
