Department of Pathology, Wayne State University School of Medicine, Detroit, MI 48202, USA.
Prostaglandins Other Lipid Mediat. 2011 Feb;94(1-2):59-72. doi: 10.1016/j.prostaglandins.2011.01.003. Epub 2011 Jan 12.
Bioactive lipids derived from polyunsaturated fatty acids constitute an important set of lipid mediators with diverse homeostatic and pathophysiological activities. The role of bioactive lipids in inflammation, active resolution of inflammation, reproduction, cardiovascular biology, chronic diseases such as cancer and metabolic disorders is an active area of research. Other than the few clinically used eicosanoids such as prostaglandin F(2)α, prostacyclin, and prostaglandin E(2), little is known about the stability of a majority of the bioactive lipids in aqueous solutions despite their extensive use under in vitro experimental conditions. The present study evaluated the stability of eicosanoids and docosanoids as well as their parent polyunsaturated fatty acids in tissue culture media with respect to incubation time, temperature, and composition of the media using multiple reaction monitoring (MRM) LC-MS. The results provide a comprehensive and valuable data set on the stability of these bioactive lipid mediators to guide in vitro experimentation.
生物活性脂质来源于多不饱和脂肪酸，构成了一组重要的脂质介质，具有多种体内平衡和病理生理活性。生物活性脂质在炎症、炎症的主动消退、生殖、心血管生物学、癌症和代谢紊乱等慢性疾病中的作用是一个活跃的研究领域。除了少数临床应用的类二十烷酸，如前列腺素 F(2)α、前列环素和前列腺素 E(2)外，尽管在体外实验条件下广泛使用，但对于大多数生物活性脂质在水溶液中的稳定性知之甚少。本研究使用多重反应监测（MRM）LC-MS 评估了类二十烷酸和二十二碳烷酸及其母体多不饱和脂肪酸在组织培养基中随孵育时间、温度和培养基成分的稳定性。研究结果提供了关于这些生物活性脂质介质稳定性的全面而有价值的数据集，以指导体外实验。
