Radionov A N, Kaulen A D
Department of Photobiochemistry, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
FEBS Lett. 1995 Dec 27;377(3):330-2. doi: 10.1016/0014-5793(95)01367-9.
The M intermediate decay in the photocycle of D96N mutant bacteriorhodopsin does not depend on the light intensity of the exciting flash. Cooperative phenomena in the photocycle are revealed after addition of azide causing acceleration of the M decay and making it kinetically well separated from the N decay. Increase in the light intensity induces slight deceleration of the M decay and significant acceleration of the N decay. The data obtained directly confirm our recent model [Komrakov and Kaulen (1995) Biophys. Chem. 56, 113-119], according to which appearance of the Mslow intermediate in the photocycle of the wild type bR at high light intensity is due to destabilization of the N intermediate leading to the acceleration of the N-->M and N-->bR reactions.
D96N突变型细菌视紫红质光循环中的M中间态衰变不依赖于激发闪光的光强度。在添加叠氮化物后，光循环中的协同现象得以显现，叠氮化物会加速M的衰变，并使其在动力学上与N的衰变很好地分离。光强度的增加会导致M衰变略有减速，而N衰变则显著加速。所获得的数据直接证实了我们最近的模型[Komrakov和Kaulen（1995年），生物物理化学杂志56卷，第113 - 119页]，根据该模型，在高光强度下野生型细菌视紫红质光循环中出现的Mslow中间态是由于N中间态的不稳定，导致N→M和N→细菌视紫红质反应加速。
