Werge T M, Biocca S, Cattaneo A
CNR Institute of Neurobiology, Roma, Italy.
FEBS Lett. 1990 Nov 12;274(1-2):193-8. doi: 10.1016/0014-5793(90)81362-r.
Following the demonstration that intracellular expression of antibodies ('intracellular immunization') may be utilized to engineer new traits in mammalian cells, we undertook experiments to perturb the function of p21ras proteins, by engineering the intracellular expression of the anti-p21ras antibody Y13-259. The variable regions of this antibody have been cloned and, after verifying their antigen binding activity, expressed in general purpose vectors for the intracellular expression of antibodies. The results confirmed that the cloned antibody has been efficiently expressed both in the secretory and the intracellular forms. Thus, intracellular immunization of mammalian cells against p21ras, or any other antigen for which a monoclonal antibody is available, can now be performed.
在证明抗体的细胞内表达（“细胞内免疫”）可用于改造哺乳动物细胞的新特性之后，我们进行了实验，通过构建抗p21ras抗体Y13-259的细胞内表达来干扰p21ras蛋白的功能。该抗体的可变区已被克隆，在验证其抗原结合活性后，在用于抗体细胞内表达的通用载体中进行表达。结果证实，克隆的抗体已以分泌形式和细胞内形式有效表达。因此，现在可以对哺乳动物细胞进行针对p21ras或任何其他有单克隆抗体可用的抗原的细胞内免疫。
