Shimura N, Komori Y, Tanaka T, Kubodera A, Oyamada H
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Int J Rad Appl Instrum B. 1990;17(6):553-60. doi: 10.1016/0883-2897(90)90131-j.
The scintigraphic imaging of mammary tumors with anti-estriol 3-sulfate (E3 3-S) antibody was studied in rats. A chemical carcinogen, 7,12-dimethylbenz(a)anthracine (DMBA), induced mammary tumors in Sprague-Dawley female rats. Highly specific anti-E3 3-S antibody was prepared and radioiodinated by [131I]NaI using the chloramine-T method. At 24 h after administration of 131I-anti-E3 3-S antibody, goat anti-guinea pig immunogloblin G (IgG) was injected as the second antibody (SA) and nuclear scintigraphy was performed. Mammary tumors were clearly visualized following SA injection.
在大鼠中研究了用抗硫酸雌三醇（E3 3-S）抗体对乳腺肿瘤进行闪烁成像。一种化学致癌物，7,12-二甲基苯并（a）蒽（DMBA），在斯普拉格-道利雌性大鼠中诱发乳腺肿瘤。制备了高度特异性的抗E3 3-S抗体，并使用氯胺-T法用[131I]NaI对其进行放射性碘化。在注射131I-抗E3 3-S抗体后24小时，注射山羊抗豚鼠免疫球蛋白G（IgG）作为第二抗体（SA）并进行核闪烁成像。注射SA后，乳腺肿瘤清晰可见。
