Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista, SP, Brazil.
Mol Cell Endocrinol. 2011 Mar 30;335(2):110-5. doi: 10.1016/j.mce.2011.01.003. Epub 2011 Jan 14.
The aim of the present study was to evaluate the effects of yerba maté extract upon markers of insulin resistance and inflammatory markers in mice with high fat diet-induced obesity. The mice were introduced to either standard or high fat diets. After 12 weeks on a high fat diet, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 gkg(-1). After treatment, glucose blood level and hepatic and soleus muscle insulin response were evaluated. Serum levels of TNF-α and IL-6 were evaluated by ELISA, liver tissue was examined to determine the mRNA levels of TNF-α, IL-6 and iNOS, and the nuclear translocation of NF-κB was determined by an electrophoretic mobility shift assay. Our data show improvements in both the basal glucose blood levels and in the response to insulin administration in the treated animals. The molecular analysis of insulin signalling revealed a restoration of hepatic and muscle insulin substrate receptor (IRS)-1 and AKT phosphorylation. Our data show that the high fat diet caused an up-regulation of the TNF-α, IL-6, and iNOS genes. Although after intervention with yerba maté extract the expression levels of those genes returned to baseline through the NF-κB pathway, these results could also be secondary to the weight loss observed. In conclusion, our results indicate that yerba maté has a potential anti-inflammatory effect. Additionally, these data demonstrate that yerba maté inhibits hepatic and muscle TNF-α and restores hepatic insulin signalling in mice with high fat diet-induced obesity.
本研究旨在评估马黛茶提取物对高脂肪饮食诱导肥胖小鼠胰岛素抵抗和炎症标志物的影响。将小鼠分别引入标准饮食或高脂肪饮食。在高脂肪饮食 12 周后，将小鼠随机分为两组，分别给予水或马黛茶提取物（1.0 gkg(-1)）。治疗后，评估血糖水平和肝、比目鱼肌胰岛素反应。通过 ELISA 评估血清 TNF-α 和 IL-6 水平，通过电泳迁移率变动分析测定肝组织 TNF-α、IL-6 和 iNOS 的 mRNA 水平，以及 NF-κB 的核易位。我们的数据显示，治疗组的基础血糖水平和胰岛素给药后的反应均有所改善。胰岛素信号的分子分析显示肝和肌肉胰岛素底物受体（IRS）-1 和 AKT 磷酸化得到恢复。我们的数据显示，高脂肪饮食导致 TNF-α、IL-6 和 iNOS 基因上调。虽然马黛茶提取物干预后，这些基因通过 NF-κB 途径恢复到基线水平，但这些结果也可能是由于观察到的体重减轻所致。总之，我们的结果表明马黛茶具有潜在的抗炎作用。此外，这些数据表明，马黛茶可抑制高脂肪饮食诱导肥胖小鼠的肝和肌肉 TNF-α，并恢复肝胰岛素信号。
