Gross I, Smith G J, Maniscalco W M, Czajka M R, Wilson C M, Rooney S A
J Appl Physiol Respir Environ Exerc Physiol. 1978 Sep;45(3):355-62. doi: 10.1152/jappl.1978.45.3.355.
We have developed a short-term organ culture model for the study of the biochemical and morphological development of late gestation fetal rat lung. Explants (1 mm3) of 19-day lung were cultured in an oxygen enriched environment in the presence of synthetic serum-free medium for 3 days. Morphological maturation continued in culture. The rate of incorporation of choline into disaturated phosphatidylcholine and the content of this phospholipid in the explants increased in vitro in a pattern very similar to that which occurs in vivo. The activities of choline kinase and cholinephosphotransferase were also similar in cultured lung and in vivo. Studies of glucose oxidation to CO2 provided additional evidence that the explants remained viable in culture. The explants retained the sensitivity of fetal lung to hormonal action. This was demonstrated by the stimulation of choline incorporation into phospholipid by cyclic AMP and an increase in the glycogen content after exposure to insulin.
我们已经开发出一种短期器官培养模型，用于研究妊娠晚期胎鼠肺的生化和形态发育。将19天龄肺的外植体（1立方毫米）在富含氧气的环境中，于合成无血清培养基存在的条件下培养3天。培养过程中形态成熟持续进行。胆碱掺入二饱和磷脂酰胆碱的速率以及外植体中这种磷脂的含量在体外增加，其模式与体内发生的模式非常相似。胆碱激酶和胆碱磷酸转移酶的活性在培养的肺和体内也相似。对葡萄糖氧化为二氧化碳的研究提供了额外证据，表明外植体在培养中保持存活。外植体保留了胎肺对激素作用的敏感性。这通过环磷酸腺苷刺激胆碱掺入磷脂以及暴露于胰岛素后糖原含量增加得以证明。
