Gross I, Freedman R M, Wilson C M, Lindsey S
Am Rev Respir Dis. 1981 Mar;123(3):313-9. doi: 10.1164/arrd.1981.123.3.313.
Monodispersed suspensions of 19-day fetal rat lung when plated onto gelatin sponges reaggregated to form alveolarlike structures. These structures consisted almost entirely of alveolar type II cells, whereas the whole cultures contained about 65% type II cells. The phospholipid content and composition of the organotypic cultures after 8 days of incubation was very similar to that of explants of 19-day fetal rat lung grown in organ culture of 48 h, as was the pattern of incorporation of choline and acetate into phospholipids. Very little phosphatidylcholine was secreted by the organotypic cultures into the culture medium. Initial observations suggested that the organ cultures are more responsive to hormones. Organotypic cultures do not appear to offer any major advantage over the simpler and quicker organ cultures for studies of hormonal influences on fetal lung maturation. They may be useful for examining the metabolism and sympathetic pathways of the type II cell.
将19天胎龄大鼠肺的单分散悬浮液接种到明胶海绵上后，会重新聚集形成肺泡样结构。这些结构几乎完全由II型肺泡细胞组成，而整个培养物中约含65%的II型细胞。培养8天后，器官型培养物的磷脂含量和组成与在器官培养中生长48小时的19天胎龄大鼠肺外植体非常相似，胆碱和乙酸掺入磷脂的模式也是如此。器官型培养物分泌到培养基中的磷脂酰胆碱极少。初步观察表明，器官型培养物对激素的反应更强。对于研究激素对胎儿肺成熟的影响，器官型培养物与更简单、更快速的器官培养相比，似乎没有任何主要优势。它们可能有助于研究II型细胞的代谢和交感神经途径。
