Environmental Toxicology Research Unit (ETRU), Pesticide Chemistry Department, National Research Centre, Cairo, Egypt.
Hum Exp Toxicol. 2011 Oct;30(10):1501-13. doi: 10.1177/0960327110391686. Epub 2011 Jan 14.
In the present study, Origanum majorana L. essential oil (EO) was analyzed by gas chromatography-mass spectrometry (GC-MS) and evaluated for free radical scavenging and anticholinesterase activities. GC-MS analysis revealed the presence of 4-terpineol (29.97%), γ-terpinene (15.40%), trans-sabinene hydrate (10.93), α-terpinene (6.86%), 3-cycolohexene-1-1 methanal,a,a4-trimethyl-,(S)-(CAS) (6.54%), and sabinene (3.91%) as main constituents. Origanum majorana L. EO exhibited concentration-dependent inhibitory effects on 2,2'-diphenylpicrylhydrazyl (DPPH(•)), hydroxyl radical, hydrogen peroxide, reducing power, and lipid peroxidation with IC(50) values of 58.67, 67.11, 91.25, 78.67, and 68.75 µg/mL, respectively; while the IC(50) values for the standard trolox were noted to be 23.95, 44.97, 51.30, 42.22, and 52.72 µg/mL, respectively. Interestingly, cholinesterase inhibitory activity was also found with IC(50) values of 36.40 µg/mL. We can conclude that the marjoram EO has a significant potential to be used as a natural antioxidant and anti-AChE.
在本研究中，分析了牛至草精油（EO），并通过气相色谱-质谱联用（GC-MS）进行了评估，以评估其自由基清除和抗胆碱酯酶活性。GC-MS 分析表明，主要成分包括 4-萜品醇（29.97%）、γ-萜品烯（15.40%）、反式-水合马鞭烯酮（10.93%）、α-萜品烯（6.86%）、3-环已烯-1-1 甲醛，a，a4-三甲基-，（S）-（CAS）（6.54%）和柠檬烯（3.91%）。牛至草精油对 2,2'-二苯基苦基肼（DPPH(•)）、羟基自由基、过氧化氢、还原能力和脂质过氧化具有浓度依赖性的抑制作用，IC50 值分别为 58.67、67.11、91.25、78.67 和 68.75 µg/mL；而标准 Trolox 的 IC50 值分别为 23.95、44.97、51.30、42.22 和 52.72 µg/mL。有趣的是，还发现了对胆碱酯酶的抑制活性，IC50 值为 36.40 µg/mL。我们可以得出结论，牛至草精油具有作为天然抗氧化剂和抗乙酰胆碱酯酶的巨大潜力。
