Ali Nasser A Awadh, Crouch Rebecca A, Al-Fatimi Mohamed A, Arnold Norbert, Teichert Axel, Setzer William N, Wessjohann Ludger
Pharmacognosy Department, Faculty of Pharmacy, Sana'a University, P. O. Box 13150, Sana'a, Yemen.
Nat Prod Commun. 2012 Jan;7(1):113-6.
The chemical composition of the hydrodistilled leaf essential oil from Pulicaria stephanocarpa Balf. Fil was determined by GC-MS analysis, and its antimicrobial, antioxidant and anticholinesterase (AChE) activities were evaluated. Eighty-three compounds were identified representing 97.2% of the total oil. (E)-Caryophyllene 13.4%, (E)-nerolidol 8.5%, caryophyllene oxide 8.5%, alpha-cadinol 8.2% spathulenol 6.8% and tau-cadinol 4.7%, were the main components. Antimicrobial activity of the oil, evaluated using the disc diffusion and broth dilution methods, demonstrated the highest susceptibility on Gram-positive bacteria and Candida albicans. The free radical scavenging ability of the oil was assessed by the DPPH assay to show antiradical activity with IC50 of 330 microg/mL. Moreover, the oil revealed an AChE inhibitory activity of 47% at a concentration of 200 microg/mL using Ellman's method.
通过气相色谱-质谱联用（GC-MS）分析确定了来自硬果和尚菜（Pulicaria stephanocarpa Balf. Fil）水蒸馏叶精油的化学成分，并评估了其抗菌、抗氧化和抗胆碱酯酶（AChE）活性。共鉴定出83种化合物，占总油含量的97.2%。主要成分包括（E）-石竹烯（13.4%）、（E）-橙花叔醇（8.5%）、石竹烯氧化物（8.5%）、α-杜松醇（8.2%）、匙叶桉油烯醇（6.8%）和τ-杜松醇（4.7%）。采用纸片扩散法和肉汤稀释法评估了该精油的抗菌活性，结果表明其对革兰氏阳性菌和白色念珠菌的敏感性最高。通过DPPH法评估了该精油的自由基清除能力，结果显示其具有抗自由基活性，IC50为330μg/mL。此外，采用埃尔曼法（Ellman's method）测定，该精油在浓度为200μg/mL时显示出47%的AChE抑制活性。
