Rayner A, Harris G, Croughan M, Watt B
Scottish Mycobacteria Reference Laboratory, City Hospital, Edinburgh.
Scott Med J. 1990 Oct;35(5):142-4. doi: 10.1177/003693309003500506.
We report the first year's experience of the use of a radiometric technique for sensitivity testing of mycobacterial isolates in Scotland. The techniques (using the Bactec 460 instrument) was much quicker than conventional methods (for example the mean time to obtain results for 60 strains of M.tuberculosis was 14.4 days, compared with 44.9 days for conventional methods). There was good agreement between results obtained by the two methods. Preliminary results also indicated the value of the radiometric technique for rapid isolation of mycobacteria from clinical samples (mean of 10.6 days with the radiometric technique compared with 26.7 days using egg media) although for optimal recovery, both techniques should be used in parallel.
我们报告了在苏格兰使用放射性技术对分枝杆菌分离株进行药敏试验的第一年经验。该技术(使用Bactec 460仪器)比传统方法快得多(例如,60株结核分枝杆菌获得结果的平均时间为14.4天,而传统方法为44.9天)。两种方法获得的结果之间具有良好的一致性。初步结果还表明放射性技术在从临床样本中快速分离分枝杆菌方面的价值(放射性技术的平均时间为10.6天,而使用鸡蛋培养基为26.7天),不过为了实现最佳回收率,两种技术应并行使用。