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吲哚连接的 C8-脱氧鸟苷核苷可作为 Watson-Crick 与 Hoogsteen 碱基配对的荧光报告基团。

An indole-linked C8-deoxyguanosine nucleoside acts as a fluorescent reporter of Watson-Crick versus Hoogsteen base pairing.

机构信息

Department of Chemistry, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

出版信息

Org Biomol Chem. 2011 Mar 7;9(5):1565-71. doi: 10.1039/c0ob00883d. Epub 2011 Jan 14.

DOI:10.1039/c0ob00883d
PMID:21240404
Abstract

Pyrrole- and indole-linked C(8)-deoxyguanosine nucleosides act as fluorescent reporters of H-bonding specificity. Their fluorescence is quenched upon Watson-Crick H-bonding to dC, while Hoogsteen H-bonding to G enhances emission intensity. The indole-linked probe is ∼ 10-fold brighter and shows promise as a fluorescent reporter of Hoogsteen base pairing.

摘要

吡咯基和吲哚基连接的 C(8)-脱氧鸟苷核苷作为氢键特异性的荧光报告基团。当与 dC 形成 Watson-Crick 氢键时，其荧光被猝灭，而与 G 形成 Hoogsteen 氢键则增强了发射强度。吲哚基探针的亮度提高了约 10 倍，有望成为 Hoogsteen 碱基配对的荧光报告基团。

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