Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, Shanghai, People's Republic of China.
IET Nanobiotechnol. 2011 Mar;5(1):14-9. doi: 10.1049/iet-nbt.2010.0001.
Three water-soluble CdTe quantum dots (QDs) (green-emitting, yellow-emitting and red-emitting) were synthesised for different refluxing time with 3-mercaptopropionic acid (MPA) as stabiliser. Then the red-emitting CdTe QDs and mouse immunoglobulin G (IgG) were taken as the representative to study the conjugation behaviour of QDs and antibody by a novel immunochromatographic method. After comparing with several methods, that is, direct conjugation, 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC)-mediated conjugation, N-hydroxysuccinimide (NHS)-mediated conjugation, EDC/NHS-mediated conjugation by immunochromatographic strips, EDC and NHS were selected together as coupling agents to conjugate QDs with antibody efficiently. Finally, the K562 leukaemia cells were incubated with the EDC/NHS-mediated conjugates to evaluate the performance in practical application, and the result from fluorescence images showed that it was successfully applied to label cells. The immunochromatographic strip was a superior method to study the conjugation of the fluorophore and antibody.
三种水溶性 CdTe 量子点（QD）（绿色发射、黄色发射和红色发射）分别用 3-巯基丙酸（MPA）作为稳定剂，在不同回流时间下合成。然后以红色发射 CdTe QD 和小鼠免疫球蛋白 G（IgG）为代表，采用一种新的免疫层析法研究 QD 与抗体的结合行为。通过比较几种方法，即直接结合、1-乙基-3(3-二甲基氨基丙基)碳化二亚胺盐酸盐（EDC）介导的结合、N-羟基琥珀酰亚胺（NHS）介导的结合、EDC/NHS 通过免疫层析条介导的结合，选择 EDC 和 NHS 作为偶联剂，有效地将 QD 与抗体偶联。最后，用 EDC/NHS 介导的缀合物孵育 K562 白血病细胞，以评估实际应用中的性能，荧光图像的结果表明，它成功地应用于标记细胞。免疫层析条是研究荧光染料与抗体结合的一种优越方法。
