基于量子点的人血清总 IgE 免疫层析检测法。
Quantum-dot-based immunochromatographic assay for total IgE in human serum.
机构信息
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
出版信息
PLoS One. 2013 Oct 30;8(10):e77485. doi: 10.1371/journal.pone.0077485. eCollection 2013.
To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.
为了快速定量检测人血清中的总免疫球蛋白 E 水平，我们开发了一种新型的基于量子点的免疫层析分析方法，该方法采用荧光数字记录。它可以检测 5-1000 kU/L 的 IgE 水平，变异系数范围为 2.0-9.5%。该测定可在 10 分钟内完成。该方法已在 95 份血清样本中进行了测试。该测定法与商业 ELISA 试剂盒测定的 IgE 值之间的相关系数为 0.9884。因此，我们的测定法有望成为一种新的 IgE 检测诊断工具。
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