Veterinary Research Institute, Brno, Czech Republic.
J Fish Dis. 2011 Feb;34(2):159-66. doi: 10.1111/j.1365-2761.2010.01224.x.
A survey was performed on ornamental fish imported into the EU to detect viral agents belonging to the genus Ranavirus. The objective was to gain knowledge of the potential for these systemic iridoviruses to gain entry into the EU via international trade in ornamental fish. A total of 208 pooled samples, representing 753 individual fish, were tested. The samples included 13 orders and 37 families, originating from different countries and continents. Tissues from fish that died during or just after transport were collected and examined by standard virological techniques in epithelioma papulosum cyprini cells, by transmission electron microscopy and by PCR for the detection of the major capsid protein and DNA polymerase gene sequences of ranaviruses. Virus was isolated from nine fish species but ranavirus was not identified in those samples. The results suggest that ranaviruses are not highly prevalent in ornamental fish imported into the EU.
对进入欧盟的观赏鱼进行了一项调查，以检测属于虹彩病毒属的病毒。目的是了解这些全身性虹彩病毒通过观赏鱼国际贸易进入欧盟的可能性。总共对 208 个混合样本，代表 753 条个体鱼进行了测试。样本包括来自不同国家和大陆的 13 个目和 37 个科。从运输过程中或运输后死亡的鱼采集组织，并通过标准的鱼类上皮瘤细胞（epithelioma papulosum cyprini cells）病毒学技术、电镜技术和 PCR 检测法进行检测，以检测虹彩病毒的主要衣壳蛋白和 DNA 聚合酶基因序列。从 9 种鱼类中分离到病毒，但在这些样本中未鉴定出虹彩病毒。结果表明，进入欧盟的观赏鱼中虹彩病毒的流行率并不高。
