Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, Guangdong Province 510380, China.
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, Guangdong Province 510380, China.
Microb Pathog. 2017 Nov;112:269-273. doi: 10.1016/j.micpath.2017.10.005. Epub 2017 Oct 5.
Ranavirus has become a noticeable threat to both farmed and natural populations of fish and amphibians. Herein, we reported that 3 strains of novel viruses, designated as ScRIV-GM-20150902, CmRIV-XT-20150917 and ScRIV-ZS-20151201, were isolated from diseased Chinese perch and snakehead fish in China. Efficient propagation of these isolates were determined in Chinese perch brain (CPB) cell line by the means of cytopathic effect observation, PCR amplification and electron microscopy observation. And their viral titers in CPB cells reached 10 TCID ml, 10 TCID ml and 10 TCID ml, respectively. While the challenge experiment results showed that 3 isolates resulted in 100% mortality of Chinese perch after virus infection. Electron microscopy analysis showed that two kinds of viral inclusion bodies (intracytoplasmic and intranuclear inclusion body) were observed in infected CPB cells. Sequence alignment and phylogenetic analysis of major capsid protein gene sequences of isolates revealed that these isolates belonged to the species Santee-Cooper Ranavirus.
虹彩病毒已成为鱼类和两栖类养殖和野生种群的显著威胁。本研究报告了从中国患病的鳜鱼和乌鳢中分离到的 3 株新型病毒，分别命名为 ScRIV-GM-20150902、CmRIV-XT-20150917 和 ScRIV-ZS-20151201。通过观察细胞病变效应、PCR 扩增和电子显微镜观察，确定了这些分离株在中华鳖脑（CPB）细胞系中的有效繁殖。它们在 CPB 细胞中的病毒滴度分别达到 10 TCID ml、10 TCID ml 和 10 TCID ml。而攻毒实验结果表明，3 株分离株感染后导致鳜鱼 100%死亡。电镜分析显示，感染 CPB 细胞中观察到两种病毒包涵体（胞质内和核内包涵体）。分离株主要衣壳蛋白基因序列的序列比对和系统发育分析表明，这些分离株属于 Santee-Cooper 虹彩病毒种。
