Guan Li, Li Zong-Yang, Zhao Jin-Yuan, Xu Xi-Xian, Wen Tao, Zhang Yan-Lin
Research Center of Occupational Medicine, The Third Hospital of Peking University, Beijing 100191, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010 Dec;28(12):885-90.
To investigate the startup detail of circulation dysfunction and its role in the progress of delayed neuropsychologic sequelae (DNS) after carbon monoxide (CO) poisoning with comparison with the model of ischemia-reperfusion.
The ischemia-reperfusion rat model was established by Pulsinelli-Brierley method, and the CO poisoning rats model by i.p. injected with CO repeatedly respectively, and the rats were identified with DNS following the experiment of pathology and the ethnology.
The whole blood viscosity, plasma viscosity, hematocrit and fibrinogen increased significantly immediately after reperfusion, and recovered gradually with the ischemia-reperfusion rat model. The whole blood viscosity decreased significantly immediately after CO treated i.p. Especially at low shear rate, the hematocrit also declined remarkably in the early stage after CO treatment. But 1day later, these parameters turned to the trend of the ischemia-reperfusion rats. There was a prominent elevation of both indexes until the 14th day following CO injection i.p.
There are significantly sustained hyper-coagulation and hyper-viscosity with circulation in rats after CO poisoning compared with ischemia-reperfusion model during the period of DNS, which might contribute to increase cerebral circulation resistance, blocked blood flow, and deteriorate hypoxemia in progression of DNS.
研究一氧化碳（CO）中毒后循环功能障碍的起始细节及其在迟发性神经精神后遗症（DNS）进展中的作用，并与缺血再灌注模型进行比较。
采用Pulsinelli-Brierley法建立缺血再灌注大鼠模型，分别经腹腔反复注射CO建立CO中毒大鼠模型，实验结束后通过病理学和行为学实验鉴定大鼠是否发生DNS。
缺血再灌注大鼠模型再灌注后即刻全血黏度、血浆黏度、血细胞比容和纤维蛋白原显著升高，并逐渐恢复。腹腔注射CO后即刻全血黏度显著降低，尤其是在低剪切速率下，CO处理后早期血细胞比容也显著下降。但1天后，这些参数呈现出与缺血再灌注大鼠相同的趋势。腹腔注射CO后直到第14天，这两个指标均显著升高。
与缺血再灌注模型相比，CO中毒大鼠在DNS期间存在显著持续的高凝状态和高黏滞循环状态，这可能导致脑循环阻力增加、血流受阻，并在DNS进展过程中使低氧血症恶化。
