Qu Ming-Yue, Deng Zhao-Hui, Zhou Zhou, Wei Ai-Min, Chen Chun-Hai, Yu Zheng-Ping, Wang Deng-Gao
Department of Military Occupational Hygiene, Third Military Medical University, Chongqing 400038, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2010 Dec;28(12):909-13.
To investigate the effects of microwave irradiation on the expression and regulation of heat shock proteins (HSPs) in primary cultured rat hippocampal neurons.
Neurons were exposed to 90 mW/cm(2) microwave irradiation for 10 minutes. Western blot was used to determine the expression of HSP27, HSP70, HSP90 and heat shock factor 1 (HSF1) at 0, 3, 6, 12 and 24 hour respectively. Real-time RT-PCR was used to determine the mRNA expression of HSF1. DNA-binding activity of HSF1 was measured by electrophoretic mobility shift assay (EMSA).
The protein expression of HSP27 was significantly increased by 22%, 36%, 18% at 3, 6, 12 h, respectively (P < 0.05). The protein expression of HSP70 was significantly increased by 23%, 32%, 26% at 3, 6, 12 h, respectively (P < 0.05, P < 0.01). The protein expression of HSP90 was significantly increased by 27%, 33% at 6, 12 h, respectively (P < 0.05, P < 0.01). The DNA-binding activity of HSF1 was stimulated, however, no significant change of the expression of HSF1 was observed on both the mRNA and protein levels.
The transcriptional activity of HSF1 is activated by microwave irradiation, which promotes the expression of HSPs. Heat shock response which contributes to establish a cytoprotective state is induced by microwave irradiation in primary cultured rat hippocampal neurons.
探讨微波辐射对原代培养大鼠海马神经元热休克蛋白（HSPs）表达及调控的影响。
将神经元暴露于90 mW/cm²的微波辐射下10分钟。分别在0、3、6、12和24小时使用蛋白质免疫印迹法检测HSP27、HSP70、HSP90和热休克因子1（HSF1）的表达。使用实时逆转录聚合酶链反应检测HSF1的mRNA表达。通过电泳迁移率变动分析（EMSA）测量HSF1的DNA结合活性。
HSP27的蛋白表达在3、6、12小时分别显著增加22%、36%、18%（P<0.05）。HSP70的蛋白表达在3、6、12小时分别显著增加23%、32%、26%（P<0.05，P<0.01）。HSP90的蛋白表达在6、12小时分别显著增加27%、33%（P<0.05，P<0.01）。HSF1的DNA结合活性受到刺激，然而，在mRNA和蛋白水平上均未观察到HSF1表达的显著变化。
微波辐射激活了HSF1的转录活性，从而促进了HSPs的表达。微波辐射在原代培养的大鼠海马神经元中诱导了热休克反应，有助于建立细胞保护状态。
