[职业性微波辐射对大鼠海马热休克蛋白70表达的影响]
[Effects of occupational microwave irradiation on heat shock protein 70 expressions in rat hippocampus].
作者信息
Hao Yu-tong, Pei Li-ping, Chen Chun-hai, Yang Xue-sen, Zhang Guang-bin, Deng Zhao-hui, Yu Zheng-ping
机构信息
Department of Military Occupational Hygiene, Third Military Medical University, Chongqing 400038, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Sep;27(9):553-6.
OBJECTIVE
To study the change of heat shock protein (HSP)70 expression after exposure to occupational microwave in rats hippocampus, and explore the role of HSP70 in the mechanism of bio-effect of microwave irradiation.
METHODS
The animal model was established by whole body exposures in 90, 5 W/cm(2) microwave irradiation field for 20 min in rats. Changes of the mRNA of hsp70 expressions in rat hippocampus at different time were studied by RT-PCR, and the protein change by Western blot.
RESULTS
The mRNA and protein expression of hsp70 in rat hippocampus increased after 90 W/cm(2) and 5 W/cm(2) microwave irradiation for 20 min. The anal temperature and the value of SAR increased significantly. These changes were positively correlated with power and irradiation time of microwave. The results indicated that microwave irradiation led to HSP70 syntheses effectively.
CONCLUSION
Microwave irradiation can obviously induce the thermal effect and activate HSP70, and initiate the endogenous protective mechanism of central nervous system.
目的
研究大鼠海马在职业性微波暴露后热休克蛋白（HSP）70表达的变化，探讨HSP70在微波辐射生物效应机制中的作用。
方法
将大鼠置于90、5 W/cm²微波辐射场中全身暴露20分钟建立动物模型。采用逆转录-聚合酶链反应（RT-PCR）研究大鼠海马不同时间hsp70表达的mRNA变化，采用蛋白质印迹法研究蛋白质变化。
结果
90 W/cm²和5 W/cm²微波辐射20分钟后，大鼠海马hsp70的mRNA和蛋白质表达增加。肛温及比吸收率（SAR）值显著升高。这些变化与微波的功率和照射时间呈正相关。结果表明微波辐射能有效诱导HSP70合成。
结论
微波辐射可明显诱导热效应并激活HSP70，启动中枢神经系统的内源性保护机制。
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