Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, The Netherlands.
Acta Biomater. 2011 May;7(5):1999-2006. doi: 10.1016/j.actbio.2011.01.018. Epub 2011 Jan 15.
At present there is a strong need for suitable scaffolds that meet the requirements for bone tissue engineering applications. The objective of this study was to investigate the suitability of porous scaffolds based on a hydroxyl functionalized polymer, poly(hydroxymethylglycolide-co-ε-caprolactone) (pHMGCL), for tissue engineering. In a recent study this polymer was shown to be a promising material for bone regeneration. The scaffolds consisting of pHMGCL or poly(ε-caprolactone) (PCL) were produced by means of a rapid prototyping technique (three-dimensional plotting) and were shown to have a high porosity and an interconnected pore structure. The thermal and mechanical properties of both scaffolds were investigated and human mesenchymal stem cells were seeded onto the scaffolds to evaluate the cell attachment properties, as well as cell viability and differentiation. It was shown that the cells filled the pores of the pHMGCL scaffold within 7 days and displayed increased metabolic activity when compared with cells cultured in PCL scaffolds. Importantly, pHMGCL scaffolds supported osteogenic differentiation. Therefore, scaffolds based on pHMGCL are promising templates for bone tissue engineering applications.
目前，人们强烈需要满足骨组织工程应用要求的合适支架。本研究的目的是研究基于羟功能化聚合物聚（羟甲基甘醇酸-co-ε-己内酯）（pHMGCL）的多孔支架在组织工程中的适用性。在最近的一项研究中，该聚合物被证明是一种有前途的骨再生材料。pHMGCL 或聚（ε-己内酯）（PCL）组成的支架是通过快速原型制造技术（三维绘图）生产的，具有高孔隙率和相互连通的孔结构。研究了两种支架的热性能和机械性能，并将人骨髓间充质干细胞接种到支架上，以评估细胞黏附特性、细胞活力和分化。结果表明，细胞在 7 天内填充了 pHMGCL 支架的孔，与在 PCL 支架中培养的细胞相比，细胞的代谢活性增加。重要的是，pHMGCL 支架支持成骨分化。因此，基于 pHMGCL 的支架是骨组织工程应用的有前途的模板。
