Xpand Biotechnology BV, MB Bilthoven, The Netherlands.
Acta Biomater. 2011 May;7(5):2007-14. doi: 10.1016/j.actbio.2011.01.017. Epub 2011 Jan 15.
To evaluate moldable osteoinductive putties for bone repair we combined microstructured biphasic calcium phosphate (BCP) particles with five different polymeric gels, carboxymethyl cellulose (CMC), Pluronic(®) F-127 (PLU), polyvinyl alcohol (PVA), chitosan (CHI) and alginate (ALG). In vitro gel dissolution showed that CMC, PLU and ALG gels dissolved rapidly (within hours), while the CHI gel took several days and the PVA gel did not dissolve within 2 weeks. Implanting the putty formulations into sheep muscle for 12 weeks demonstrated ectopic bone formation in the control BCP group as well as the putties prepared with dissolving gels (CMC, PLU, ALG and CHI). Bone was not seen in the putty comprising PVA. Quantitative data showed that the CMC and PLU gels did not significantly affect the osteoinductivity of BCP granules, while the ALG and CHI gels showed a significant decrease in bone formation. These results suggest that the dissolvability and chemistry of the gels may be factors affecting the osteoinduction of the putties.
为了评估可塑成骨诱导性糊剂在骨修复中的应用，我们将具有微观结构的双相磷酸钙（BCP）颗粒与五种不同的聚合物凝胶（羧甲基纤维素（CMC）、泊洛沙姆 F-127（PLU）、聚乙烯醇（PVA）、壳聚糖（CHI）和藻酸盐（ALG））结合起来。体外凝胶溶解实验表明，CMC、PLU 和 ALG 凝胶迅速溶解（数小时内），而 CHI 凝胶需要数天，PVA 凝胶在 2 周内未溶解。将糊剂配方植入绵羊肌肉 12 周后，在对照 BCP 组以及溶解凝胶（CMC、PLU、ALG 和 CHI）制备的糊剂中均观察到异位骨形成。在包含 PVA 的糊剂中未观察到骨。定量数据表明，CMC 和 PLU 凝胶对 BCP 颗粒的成骨诱导作用没有显著影响，而 ALG 和 CHI 凝胶则显示出骨形成显著减少。这些结果表明，凝胶的可溶解性和化学性质可能是影响糊剂成骨诱导的因素。
