氨基酸的荧光硫代乙内酰脲和二甲基氨基偶氮苯硫代乙内酰脲衍生物的毛细管区带电泳分离及基于激光的检测
Capillary zone electrophoresis separation and laser-based detection of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids.
作者信息
Waldron K C, Wu S L, Earle C W, Harke H R, Dovichi N J
机构信息
Department of Chemistry, University of Alberta, Edmonton, Canada.
出版信息
Electrophoresis. 1990 Sep;11(9):777-80. doi: 10.1002/elps.1150110918.
Capillary zone electrophoresis is employed for the separation and analysis of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids. Detection of minute amounts of these amino acid derivatives is an important milestone in the development of a high sensitivity protein sequencer. Current detection limits for the fluorescein derivative is on the order of 10(-21) moles whereas detection limits for the dimethylaminoazobenzene derivative is on the order of 10(-16) moles.
毛细管区带电泳用于氨基酸的荧光硫代乙内酰脲和二甲基氨基偶氮苯硫代乙内酰脲衍生物的分离与分析。检测这些微量氨基酸衍生物是高灵敏度蛋白质测序仪发展中的一个重要里程碑。目前荧光素衍生物的检测限约为10^(-21)摩尔，而二甲基氨基偶氮苯衍生物的检测限约为10^(-16)摩尔。
Zotero 插件