Cheng Y F, Dovichi N J
Department of Chemistry, University of Alberta, Edmonton, Canada.
Science. 1988 Oct 28;242(4878):562-4. doi: 10.1126/science.3140381.
Subattomole analysis of fluorescein isothiocyanate (FITC) derivatives of amino acids is accomplished by combining capillary zone electrophoresis for high-efficiency separation with laser-induced fluorescence for high-sensitivity detection. Concentration detection limits range from 5 x 10(-12) molar for alanine to 9 x 10(-11) molar for lysine, injected in the column; 9 x 10(-21) mole of alanine is contained within the approximately 1-nanoliter injection volume at the detection limit. The alanine detection limit corresponds to fewer than 6000 molecules injected onto the column and represents an improvement of four orders of magnitude in the state of the art for fluorescent detection of amino acids and an improvement of six orders of magnitude in the state of the art for the detection limit for isothiocyanate derivatives of amino acids.
通过将用于高效分离的毛细管区带电泳与用于高灵敏度检测的激光诱导荧光相结合，实现了对氨基酸异硫氰酸荧光素（FITC）衍生物的亚阿托摩尔分析。注入柱中的浓度检测限范围从丙氨酸的5×10⁻¹²摩尔到赖氨酸的9×10⁻¹¹摩尔；在检测限下，约1纳升的进样体积中含有9×10⁻²¹摩尔的丙氨酸。丙氨酸的检测限对应于注入柱中的分子数少于6000个，代表了氨基酸荧光检测技术水平提高了四个数量级，以及氨基酸异硫氰酸酯衍生物检测限技术水平提高了六个数量级。
