Kokoza E B, Kozlova T Iu, Umbetova G Kh, Dubrovskiĭ E B, Pirrotta V, Zhimulev I F
Genetika. 1990 Aug;26(8):1361-9.
With the help of in situ hybridization respective location of 33 microclones from the genomic library of the Drosophila melanogaster large band 10A1-2, 4 clones obtained by chromosomal walking and 18 chromosomal rearrangements with breakpoints in the limits of this band was determined. A DNA fragment homologous to poly(A)+ RNA from the 3rd instar larvae has been revealed. Summarizing the obtained and published data, at least 3 genes and 6 transcriptionally active fragments appear to be located in the 10A1-2 band. Using DNA clones from different regions of Drosophila melanogaster 10A1-2 as probes in some Diptera species, the 10A1-2 distal clone, carrying vermilion gene, in D. virilis was shown to be located in a very thin band of the 5A region, while the proximal clones of the 10A1-2 band were mapped in a large band of the 2B region. In D. paranaensis a sequence homologous to the vermilion gene DNA was mapped in a large band, whereas the proximal clones of the 10A1-2 band were localized in a different region. These results evidence for the fact that DNA sequences are not evolutionary fixed, i. e. in different species they may belong to different chromomeres.
借助原位杂交技术，确定了来自黑腹果蝇大带10A1 - 2基因组文库的33个微克隆、通过染色体步移获得的4个克隆以及18个断点位于该带范围内的染色体重排的各自位置。已揭示出与三龄幼虫的聚腺苷酸加RNA（poly(A)+ RNA）同源的DNA片段。综合已获得的数据和已发表的数据，至少3个基因和6个转录活性片段似乎位于10A1 - 2带中。使用来自黑腹果蝇10A1 - 2不同区域的DNA克隆作为一些双翅目物种的探针，携带朱红色基因（vermilion gene）的10A1 - 2远端克隆在粗壮果蝇（D. virilis）中显示位于5A区域的一条非常细的带中，而10A1 - 2带的近端克隆则定位在2B区域的一条大带中。在巴拉那果蝇（D. paranaensis）中，与朱红色基因DNA同源的序列定位在一条大带中，而10A1 - 2带的近端克隆则位于不同区域。这些结果证明了DNA序列并非在进化上固定不变，即它们在不同物种中可能属于不同的染色粒。
