Department of Bioinformatics and Computational Biology, University of Medical Sciences in Poznan, Poznan, Poland.
J Clin Pathol. 2011 Jul;64(7):626-30. doi: 10.1136/jcp.2010.086553. Epub 2011 Jan 17.
The aim was to assess changes of B and T lymphocytes and selected apoptotic markers in Hashimoto thyroiditis (HT) cases on the basis of quantitative immunohistochemical studies (CD20, CD43, CD8, Bcl-2, caspase-3). The control group comprised colloid goitres without inflammatory infiltrate taken from 10 female patients. Thyroid specimens were obtained retrospectively from 40 patients. The immunohistochemical reactions were subject to quantitative evaluation performed using image-processing methods, including a spatial visualisation of the markers' expression. The percentage of Bcl-2 reactions in HT (mean 3.65%, SD 2.94%) was significantly lower than in the control group (mean 13.99%, SD 5.04%), while the thyroid follicles in HT samples exhibited a higher degree of staining for caspase-3 (mean 1.10%, SD 1.03%) in contrast to normal control tissues (mean 0.48%, SD 1.02%). The results from this study indicate that apoptosis plays a major role in the patogenesis of autoimmune thyroid diseases containing the main pathogenic events in the lesion of thyroid follicular cells in HT. Moreover, the reactivity of CD43 and CD20 was significantly higher in Hashimoto disease, while CD8 was not significantly different from the control group.
目的是通过定量免疫组织化学研究（CD20、CD43、CD8、Bcl-2、caspase-3）评估桥本甲状腺炎（HT）患者 B 和 T 淋巴细胞以及选定的凋亡标志物的变化。对照组由 10 名女性患者的无炎症浸润的胶体甲状腺肿组成。甲状腺标本从 40 名患者中回顾性获得。免疫组织化学反应采用图像处理方法进行定量评估，包括标记物表达的空间可视化。HT 中的 Bcl-2 反应百分比（平均值 3.65%，标准差 2.94%）明显低于对照组（平均值 13.99%，标准差 5.04%），而 HT 样本中的甲状腺滤泡对 caspase-3 的染色程度更高（平均值 1.10%，标准差 1.03%）与正常对照组织（平均值 0.48%，标准差 1.02%）相比。这项研究的结果表明，细胞凋亡在包含 HT 甲状腺滤泡细胞病变中主要致病事件的自身免疫性甲状腺疾病的发病机制中起主要作用。此外，CD43 和 CD20 在桥本氏病中的反应性明显更高，而 CD8 与对照组无显著差异。
