Shanmugasundaram Revathi, Selvaraj Ramesh K
Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
J Immunol. 2011 Feb 15;186(4):1997-2002. doi: 10.4049/jimmunol.1002040. Epub 2011 Jan 17.
Chicken CD4(+)CD25(+) cells were characterized for mammalian regulatory T cells' suppressive and cytokine production properties. Anti-chicken CD25 mAb was produced in mice and conjugated with a fluorescent tag. The specificity of the Ab against chicken CD25 was confirmed by evaluating Con A-induced CD25 upregulation in thymocytes and by quantifying the CD25 mRNA content of positive and negative cells identified by anti-chicken CD25 Ab. The percentage of CD4(+)CD25(+) cells, expressed as a percentage of CD4(+) cells, in thymus and blood was ∼3-7%, in spleen was 10%, and in cecal tonsil, lung, and bone marrow was ∼15%. Bursa had no detectable CD4(+)CD25(+) cells. CD25(+) cells were mostly CD4(+) in the thymus, whereas in every other organ studied, CD25(+) cells were distributed between CD4(+) and CD4(-) cells. Chicken thymic CD4(+)CD25(+) cells did not proliferate in vitro in the absence of recombinant chicken IL-2 (rCIL-2). In the presence of rCIL-2, PMA plus ionomycin or Con A stimulated CD4(+)CD25(+) cell proliferation, whereas anti-CD3 plus CD28 did not stimulate CD4(+)CD25(+) cell proliferation. Naive CD4(+)CD25(+) cells had 29-fold more IL-10 mRNA and 15-fold more TGF-β mRNA than the naive CD4(+)CD25(-) cells. Naive CD4(+)CD25(+) had no detectable IL-2 mRNA. Both naive and PMA plus ionomycin-stimulated thymic CD4(+)CD25(+) cells suppressed naive T cell proliferation. The suppressive properties were partially contact dependent. Supplementing CD4(+)CD25(+) cell coculture with rCIL-2 reversed the suppressive properties of CD4(+)CD25(+) cells. Chicken CD4(+)CD25(+) cells have suppressive properties similar to that of mammalian regulatory T cells.
对鸡的CD4(+)CD25(+)细胞进行了表征，以了解其具有哺乳动物调节性T细胞的抑制特性和细胞因子产生特性。抗鸡CD25单克隆抗体在小鼠体内产生，并与荧光标签偶联。通过评估刀豆蛋白A诱导胸腺细胞中CD25上调情况以及定量抗鸡CD25抗体鉴定出的阳性和阴性细胞的CD25 mRNA含量，证实了该抗体对鸡CD25的特异性。胸腺和血液中CD4(+)CD25(+)细胞占CD4(+)细胞的百分比约为3 - 7%，脾脏中为10%，盲肠扁桃体、肺和骨髓中约为15%。法氏囊中未检测到CD4(+)CD25(+)细胞。胸腺中CD25(+)细胞大多为CD4(+)，而在其他所有研究的器官中，CD25(+)细胞分布于CD4(+)和CD4(-)细胞之间。在没有重组鸡白细胞介素-2（rCIL-2）的情况下，鸡胸腺CD4(+)CD25(+)细胞在体外不增殖。在rCIL-2存在的情况下，佛波酯加离子霉素或刀豆蛋白A可刺激CD4(+)CD25(+)细胞增殖，而抗CD3加CD28则不能刺激CD4(+)CD25(+)细胞增殖。初始CD4(+)CD25(+)细胞的白细胞介素-10 mRNA比初始CD4(+)CD25(-)细胞多29倍，转化生长因子-β mRNA多15倍。初始CD4(+)CD25(+)细胞未检测到白细胞介素-2 mRNA。初始的以及经佛波酯加离子霉素刺激的胸腺CD4(+)CD25(+)细胞均抑制初始T细胞增殖。抑制特性部分依赖细胞接触。用rCIL-2补充CD4(+)CD25(+)细胞共培养可逆转CD4(+)CD25(+)细胞的抑制特性。鸡CD4(+)CD25(+)细胞具有与哺乳动物调节性T细胞相似的抑制特性。
