Fatemi Seyed Abolghasem, Levy April Waguespack, Peebles Edgar David
Department of Poultry Science, Mississippi State University, MS 39762, USA.
DSM Nutritional Products, Parsippany, NJ 07054, USA.
Animals (Basel). 2024 Dec 24;15(1):10. doi: 10.3390/ani15010010.
Effects of and dietary sources of calcidiol (25(OH)D), combined with Marek's disease vaccine (MDV), on the expression of genes involved with the antioxidant activity, muscle deposition, and immunity in the pectoralis major (P. major) muscle and spleen of 40 d of age (doa) broilers were investigated. The treatments were as follows: (1) non-injected; (2) the injection of 50 μL of commercial MDV, (3) MDV + 1.2, or (4) 2.4 μg of 25(OH)D. All birds received either a commercial diet containing no supplemental 25(OH)D (control) or the same diet supplemented with an additional 69 µg of 25(OH)D per kg of feed (Hy-D diet). At 40 doa, the pectoralis major (P. major) muscle and spleen of 48 birds (six replicates per diet x treatment combination) were collected. When compared to un-supplemented commercial diet-fed birds, in birds that were fed the Hy-D diet, the expression of the TGF-β4 gene in the spleen and P. major muscle, and the , , , , , and genes in the P. major muscle were up-regulated, whereas the expression of the , , and genes in the spleen and P. major muscle were down-regulated. Nevertheless, birds that received any of the injection doses of 25(OH)D exhibited a higher expression of the , , and genes in the spleen and P. major muscle. Furthermore, in comparison to the MDV-injected control group, the , , and genes in the P. major muscle were up-regulated, and the expression of the , and genes in the spleen and the , and genes in the P. major muscle were down-regulated. In conclusion, a significant improvement in the expression of genes responsible for enzymatic antioxidant activity, protein synthesis, and inflammatory reactions in 40-day-old broilers occurred in response to and dietary supplemental 25(OH)D, and supplemental 25OHD provided via either route was used to enhance the expression of genes linked to vitamin D activity (, ).
研究了骨化二醇（25(OH)D）及其膳食来源与马立克氏病疫苗（MDV）联合使用对40日龄肉鸡胸大肌和脾脏中与抗氧化活性、肌肉沉积及免疫相关基因表达的影响。处理方式如下：（1）未注射；（2）注射50 μL商业MDV；（3）MDV + 1.2 μg；或（4）2.4 μg 25(OH)D。所有鸡只均饲喂不含额外添加25(OH)D的商业日粮（对照组）或每千克饲料额外添加69 μg 25(OH)D的相同日粮（Hy-D日粮）。在40日龄时，收集48只鸡（每种日粮×处理组合6个重复）的胸大肌和脾脏。与未添加商业日粮的鸡相比，饲喂Hy-D日粮的鸡脾脏和胸大肌中TGF-β4基因以及胸大肌中 、 、 、 、 和 基因的表达上调，而脾脏和胸大肌中 、 和 基因的表达下调。然而，接受任何注射剂量25(OH)D的鸡脾脏和胸大肌中 、 和 基因的表达较高。此外，与MDV注射对照组相比，胸大肌中 、 和 基因上调，脾脏中 、 和 基因以及胸大肌中 、 和 基因的表达下调。总之，40日龄肉鸡中负责酶促抗氧化活性、蛋白质合成和炎症反应的基因表达因25(OH)D及其膳食补充剂而显著改善，通过任何一种途径提供的补充25OHD均用于增强与维生素D活性相关基因（ 、 ）的表达。
