Greenberg S, Burridge K, Silverstein S C
Rover Laboratory of Cellular Physiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
J Exp Med. 1990 Dec 1;172(6):1853-6. doi: 10.1084/jem.172.6.1853.
We have studied the distribution of talin in J774 cells and mouse peritoneal macrophages undergoing Fc receptor-mediated phagocytosis. At early stages of phagocytosis, talin accumulates in the cells' cortical cytoplasm adjacent to the forming phagosome and extends into pseudopods that are encircling the particle. Talin colocalizes with F-actin at these sites. After particle ingestion is completed, F-actin and talin are no longer concentrated adjacent to phagosomes. Thus, talin and F-actin undergo dynamic and coordinate changes in their cytoplasmic location during Fc receptor-mediated phagocytosis.
我们研究了踝蛋白在J774细胞和经历Fc受体介导吞噬作用的小鼠腹腔巨噬细胞中的分布。在吞噬作用的早期阶段，踝蛋白积聚在靠近正在形成的吞噬体的细胞皮质细胞质中，并延伸到环绕颗粒的伪足中。踝蛋白在这些部位与F-肌动蛋白共定位。颗粒摄取完成后，F-肌动蛋白和踝蛋白不再集中在吞噬体附近。因此，在Fc受体介导的吞噬作用过程中，踝蛋白和F-肌动蛋白在细胞质中的位置发生动态和协同变化。
