外周血γ/δ T细胞中孔形成蛋白的组成性表达：对其体内细胞毒性作用的影响。
Constitutive expression of pore-forming protein in peripheral blood gamma/delta T cells: implication for their cytotoxic role in vivo.
作者信息
Nakata M, Smyth M J, Norihisa Y, Kawasaki A, Shinkai Y, Okumura K, Yagita H
机构信息
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
出版信息
J Exp Med. 1990 Dec 1;172(6):1877-80. doi: 10.1084/jem.172.6.1877.
Abstract
The cytotoxic activity and pore-forming protein (PFP) expression of human peripheral blood (PB) gamma/delta T cells were examined. Fresh gamma/delta T cells isolated from PB lymphocytes by fluorescence-activated cell sorting exhibited a substantial natural killer-like cytotoxic activity against K562 target cells and had a high cytotoxic potential triggered by anti-CD3 monoclonal antibody (mAb) against P815 target cells bearing Fc gamma R. Immunocytochemical staining with an anti-PFP mAb revealed that virtually all PB gamma/delta T cells are granular lymphocytes with abundant PFP in their cytoplasmic granules. Constitutive expression of PFP in PB gamma/delta T cells was also demonstrated by Northern blot analysis. These observations support the proposed role of gamma/delta T cells in cytolytic immune surveillance in vivo.
摘要
检测了人外周血（PB）γ/δT细胞的细胞毒性活性和孔形成蛋白（PFP）表达。通过荧光激活细胞分选从PB淋巴细胞中分离出的新鲜γ/δT细胞对K562靶细胞表现出显著的自然杀伤样细胞毒性活性，并且对携带FcγR的P815靶细胞具有由抗CD3单克隆抗体（mAb）触发的高细胞毒性潜力。用抗PFP mAb进行免疫细胞化学染色显示，几乎所有PBγ/δT细胞都是颗粒淋巴细胞，其细胞质颗粒中含有丰富的PFP。Northern印迹分析也证实了PBγ/δT细胞中PFP的组成性表达。这些观察结果支持了γ/δT细胞在体内溶细胞免疫监视中的作用。
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