Nakata M, Kawasaki A, Azuma M, Tsuji K, Matsuda H, Shinkai Y, Yagita H, Okumura K
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Int Immunol. 1992 Sep;4(9):1049-54. doi: 10.1093/intimm/4.9.1049.
To verify the physiological role of the pore-forming protein perforin in vivo, its expression in subpopulations of human peripheral blood lymphocytes was examined by immunocytochemical staining and their cytolytic potentials compared. In addition to NK cells and gamma delta T cells, which uniformly expressed abundant perforin in their cytoplasmic granules, only a small subpopulation of CD8+ alpha beta T cells contained perforin, namely the CD11b+ subset. However, in vitro activation with an anti-CD3 antibody and IL-2 induced perforin expression in approximately 50% of the CD8+CD11b- T cells and also in a small subset of CD4+ T cells. A distribution of perforin in CD8+ and CD4+ T cells, similar to in vitro activated T cells, was observed in fresh peripheral blood lymphocytes from infectious mononucleosis patients. In all instances, the expression of perforin correlated with the cytolytic potential of these subpopulations. The results strongly suggest that perforin plays a role in the manifestation of cytotoxic activity in vivo.
为了在体内验证成孔蛋白穿孔素的生理作用，通过免疫细胞化学染色检测了其在人外周血淋巴细胞亚群中的表达，并比较了它们的细胞溶解潜能。除了在细胞质颗粒中均匀表达丰富穿孔素的自然杀伤细胞（NK细胞）和γδT细胞外，只有一小部分CD8⁺αβT细胞含有穿孔素，即CD11b⁺亚群。然而，用抗CD3抗体和白细胞介素-2进行体外激活可诱导约50%的CD8⁺CD11b⁻T细胞以及一小部分CD4⁺T细胞表达穿孔素。在传染性单核细胞增多症患者的新鲜外周血淋巴细胞中观察到穿孔素在CD8⁺和CD4⁺T细胞中的分布，类似于体外激活的T细胞。在所有情况下，穿孔素的表达与这些亚群的细胞溶解潜能相关。结果强烈表明穿孔素在体内细胞毒性活性的表现中起作用。
