Department of Pediatrics, Taipei Veterans General Hospital, Taiwan, ROC. yjchan @ vghtpe.gov.tw
Intervirology. 2011;54(4):196-201. doi: 10.1159/000322382. Epub 2011 Jan 14.
To investigate the differences of genotypic distributions among isolates between immunosuppressed and immunocompetent patients in a Taiwanese population.
Human cytomegalovirus (HCMV) isolates from 76 patients with adequate chart data were analyzed. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to define gB genotypes which include gB1 to gB4. The clinical data of the 76 patients were retrospectively collected by chart review and classified into an immunosuppressed (n = 32) or immunocompetent (n = 44) group.
Among the 32 immunosuppressed patients, the most commonly identified HCMV genotypes were gB1 (27/32, 84.3%) and gB3 (4/32, 12.5%). 59.1% (26/44) of the immunocompetent patients were infected by gB1 while 38.6% (17/44) of them were infected by gB3. The frequency of gB1 infection in the immunosuppressed group was significantly higher than that in the immunocompetent group (p = 0.025). However, there was no statistically significant difference between gB1 and gB3 distributions by clinical diagnosis within each group.
Only gB1 and gB3 genotypes were identified in this Taiwanese population. Although there is no significant difference between clinical diagnosis and gB genotyping, gB1 infection is significantly more predominant in immunosuppressed patients.
在台湾人群中，研究免疫抑制和免疫功能正常患者之间分离株基因型分布的差异。
分析了 76 例具有充分图表数据的患者的人类巨细胞病毒（HCMV）分离株。聚合酶链反应-限制性片段长度多态性（PCR-RFLP）用于定义 gB 基因型，包括 gB1 至 gB4。通过病历回顾收集了 76 例患者的临床数据，并分为免疫抑制组（n=32）和免疫功能正常组（n=44）。
在 32 例免疫抑制患者中，最常见的 HCMV 基因型是 gB1（27/32，84.3%）和 gB3（4/32，12.5%）。59.1%（26/44）的免疫功能正常患者感染 gB1，而 38.6%（17/44）的患者感染 gB3。免疫抑制组 gB1 感染的频率明显高于免疫功能正常组（p=0.025）。然而，在每个组中，根据临床诊断，gB1 和 gB3 的分布没有统计学差异。
在台湾人群中仅鉴定出 gB1 和 gB3 基因型。尽管临床诊断和 gB 基因分型之间没有显著差异，但 gB1 感染在免疫抑制患者中明显更为普遍。
