先天性感染新生儿中的人巨细胞病毒糖蛋白B基因型
[Human cytomegalovirus glycoprotein B genotypes in congenitally infected neonates].
作者信息
Zhang Min-gang, Wang Heng-bing, Wang Yan-zhou, Pan Qi
机构信息
Department of Pediatric Orthopedic, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Aug;25(4):262-4.
OBJECTIVE
To investigate human cytomegalovirus (HCMV) glycoprotein B (gB) genotypes and clinical features in neonates with congenital infections.
METHODS
Urine samples were obtained from 67 neonates with HCMV infection confirmed by polymerase chain reaction (PCR). The gB gene fragment was amplified by nested PCR. HCMV gB genotyping was detected by restriction fragment length polymorphism.
RESULTS
In all these cases, the most prevalent genotype was gBl (50.7%), followed by gB3 (23.9%), gB2 (17.9%), and gBl/gB3 coinfection (7.5%); gB4 was not found. Moreover, gB1 was more prevalent in infants with liver damage (27/37, 73.0%) than in other symptomatic infants without liver damage (13/30, 43.3%; P < 0.05).
CONCLUSION
The gBI genotype is the most prevalent in infants with congenital symptomatic HCMV disease, especially in those with liver damage, followed by genotypes gB3, gB2, and gB4.
目的
研究先天性感染新生儿的人巨细胞病毒（HCMV）糖蛋白B（gB）基因型及临床特征。
方法
收集67例经聚合酶链反应（PCR）确诊为HCMV感染的新生儿尿液样本。采用巢式PCR扩增gB基因片段。通过限制性片段长度多态性检测HCMV gB基因分型。
结果
在所有这些病例中，最常见的基因型是gB1（50.7%），其次是gB3（23.9%）、gB2（17.9%）和gB1/gB3合并感染（7.5%）；未发现gB4。此外，gB1在有肝损伤的婴儿中（27/37，73.0%）比在其他无肝损伤的有症状婴儿中（13/30，43.3%；P<0.05）更常见。
结论
gB1基因型在先天性有症状HCMV疾病的婴儿中最常见，尤其是在有肝损伤的婴儿中，其次是gB3、gB2和gB4基因型。
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