Przegaliński E, Jaworska L, Gołembiowska K
Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Neuropeptides. 1990 Apr;15(4):195-200. doi: 10.1016/0143-4179(90)90013-o.
The effect of fenfluramine (20 mg/kg i.p.) was studied on the thyrotropin-releasing hormone (TRH) content in several brain structures and the ventral part of the lumbar spinal cord of the rat. The effect of fenfluramine on the concentration of 5-hydroxytryptamine (5-HT) in some brain structures and the lumbar spinal cord was also examined. It was found that fenfluramine had no effect on the TRH level in the hypothalamus, hippocampus, occipital cortex, septum, nucleus accumbens and ventral part of the lumbar spinal cord, though the drug produced a profound depletion (by more than 60%) of 5-HT in the hypothalamus, nucleus accumbens, striatum and lumbar spinal cord. On the other hand, fenfluramine significantly increased the TRH content in the striatum, this effect was completely abolished by citalopram (20 mg/kg i.p.) or metergoline (10 mg/kg i.p.) Citalopram also prevented the fenfluramine-induced depletion of the striatal 5-HT. These results indicate a separate neuronal storage of TRH and 5-HT in the structures (ventral part of the lumbar spinal cord, nucleus accumbens, septum) in which the peptide and indoleamine coexist in 5-HT neurons. They also suggest that the fenfluramine-induced increase in the striatal TRH concentration is due to 5-HT release and stimulation of 5-HT receptors.
研究了芬氟拉明（腹腔注射20毫克/千克）对大鼠几种脑结构和腰脊髓腹侧部促甲状腺激素释放激素（TRH）含量的影响。还检测了芬氟拉明对一些脑结构和腰脊髓中5-羟色胺（5-HT）浓度的影响。结果发现，芬氟拉明对下丘脑、海马体、枕叶皮质、隔区、伏隔核和腰脊髓腹侧部的TRH水平没有影响，尽管该药物使下丘脑、伏隔核、纹状体和腰脊髓中的5-HT显著减少（超过60%）。另一方面，芬氟拉明显著增加了纹状体中的TRH含量，西酞普兰（腹腔注射20毫克/千克）或美替拉林（腹腔注射10毫克/千克）可完全消除这种作用。西酞普兰还可防止芬氟拉明诱导的纹状体5-HT耗竭。这些结果表明，在5-HT神经元中肽和吲哚胺共存的结构（腰脊髓腹侧部、伏隔核、隔区）中，TRH和5-HT在神经元中是分开储存的。它们还表明，芬氟拉明诱导的纹状体TRH浓度增加是由于5-HT释放和5-HT受体刺激所致。
