Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada.
Biomed Microdevices. 2011 Apr;13(2):393-401. doi: 10.1007/s10544-010-9508-0.
Laser scanning cytometry (LSC) is emerging as a clinical tool. In one application a "Clatch" slide, named after the inventor, is used in conjunction with LSC for cell surface marker immunophenotyping of patient samples. The slide requires time consuming and laborious pipetting steps, making a test tedious and prone to handling errors. The Clatch slide also uses a significant number of cells, limiting the number of analyses on paucicellular samples. This paper presents an automated microfluidic system consisting of a control circuit, a microfluidic system, and an aluminum frame, capable of performing immunophenotyping procedures. This prototype system reduces 36 pipetting steps to 1, reduces the amount of cell sample from 180 μL to 56 μL, and shortens the time used by technicians.
激光扫描细胞仪（LSC）正逐渐成为一种临床工具。在一项应用中，一种名为“Clatch”的载玻片与 LSC 结合使用，用于对患者样本进行细胞表面标志物免疫表型分析。该载玻片需要耗时费力的移液步骤，使测试繁琐且容易出现操作失误。Clatch 载玻片还使用了大量的细胞，限制了对细胞数量较少的样本的分析数量。本文介绍了一种由控制电路、微流控系统和铝制框架组成的自动化微流控系统，能够执行免疫表型分析程序。该原型系统将 36 个移液步骤减少到 1 个，将细胞样本量从 180μL 减少到 56μL，并缩短了技术人员的用时。
