Department of Immunobiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
J Investig Allergol Clin Immunol. 2010;20(6):463-8.
Schefflera leucantha Viguier is used as a traditional medicine in Thailand and China to relieve chronic cough and asthma. However, little is known about its anti-allergic effects.
This study was designed to investigate the effects of S leucantha ethanol extract (SLEE) on chemokine production by epidermal Langerhans cells (LCs) stimulated with peptidoglycan (PEG) from Staphylococcus aureus and histamine release from mast cells.
LCs were purified from murine epidermal cells using the panning method with anti-IA(d) monoclonal antibody. Chemokine production by LCs was investigated by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA). Mast cells for histamine release assay were induced by long-term culture of mouse spleen cells. Histamine release from these mast cells was measured by a competitive ELISA.
Production of the eosinophil chemoattractant CCL5 and the type 2 T helper (TH2)-associated chemokine CCL17 from PEG-stimulated LCs was significantly inhibited by SLEE. Furthermore, SLEE significantly decreased the release of histamine from mast cells by IgE-mediated degranulation.
These results suggest that S leucantha may offer a new therapeutic approach for the control of atopic dermatitis associated with S aureus colonization through inhibition of the production of allergic mediators.
Schefflera leucantha Viguier 在泰国和中国被用作传统药物，用于缓解慢性咳嗽和哮喘。然而，其抗过敏作用知之甚少。
本研究旨在研究 Schefflera leucantha 乙醇提取物（SLEE）对金黄色葡萄球菌肽聚糖（PEG）刺激的表皮朗格汉斯细胞（LC）趋化因子产生和肥大细胞组胺释放的影响。
采用抗 IA(d)单克隆抗体的淘选法从鼠表皮细胞中纯化 LC。通过逆转录聚合酶链反应和酶联免疫吸附试验（ELISA）研究 LC 的趋化因子产生。用于组胺释放测定的肥大细胞通过长期培养鼠脾细胞诱导。通过竞争性 ELISA 测定这些肥大细胞的组胺释放。
SLEE 显著抑制 PEG 刺激的 LC 产生嗜酸性粒细胞趋化因子 CCL5 和 2 型 T 辅助（TH2）相关趋化因子 CCL17。此外，SLEE 显著减少了 IgE 介导的脱颗粒肥大细胞释放组胺。
这些结果表明，S. leucantha 可能通过抑制过敏介质的产生，为控制金黄色葡萄球菌定植相关特应性皮炎提供一种新的治疗方法。
