Wirotesangthong Mali, Inagaki Naoki, Tanaka Hiroyuki, Thanakijcharoenpath Witchuda, Nagai Hiroichi
Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int Immunopharmacol. 2008 Mar;8(3):453-7. doi: 10.1016/j.intimp.2007.11.005. Epub 2007 Dec 4.
The leaves of the Piper betle Linn. (Piperaceae) are used in traditional medicine and possess anti-oxidant, anti-bacterial, anti-fungal, anti-diabetic and radioprotective activities. However, little is known about their anti-allergic activity. Therefore, the effects of P. betle ethanolic extract (PE) on the production of histamine and granulocyte macrophage-colony-stimulating factor (GM-CSF) by murine bone marrow mast cells (BMMCs) and on the secretion of eotaxin and IL-8 by the human lung epithelial cell line, BEAS-2B, were investigated in vitro. PE significantly decreased histamine and GM-CSF produced by an IgE-mediated hypersensitive reaction, and inhibited eotaxin and IL-8 secretion in a TNF-alpha and IL-4-induced allergic reaction. The results suggest that P. betle may offer a new therapeutic approach for the control of allergic diseases through inhibition of production of allergic mediators.
蒌叶（胡椒科）的叶子被用于传统医学，具有抗氧化、抗菌、抗真菌、抗糖尿病和辐射防护活性。然而，人们对其抗过敏活性知之甚少。因此，体外研究了蒌叶乙醇提取物（PE）对小鼠骨髓肥大细胞（BMMCs）组胺和粒细胞巨噬细胞集落刺激因子（GM-CSF）产生以及对人肺上皮细胞系BEAS-2B嗜酸性粒细胞趋化因子和IL-8分泌的影响。PE显著降低了由IgE介导的超敏反应产生的组胺和GM-CSF，并在TNF-α和IL-4诱导的过敏反应中抑制了嗜酸性粒细胞趋化因子和IL-8的分泌。结果表明，蒌叶可能通过抑制过敏介质的产生为控制过敏性疾病提供一种新的治疗方法。
