NdhP 和 NdhQ：两种新型的蓝细菌 NDH-1 复合物的小亚基。
NdhP and NdhQ: two novel small subunits of the cyanobacterial NDH-1 complex.
机构信息
Department of Plant Biochemistry, Ruhr-University Bochum, D-44780 Bochum, Germany.
出版信息
Biochemistry. 2011 Feb 22;50(7):1121-4. doi: 10.1021/bi102044b. Epub 2011 Jan 27.
Abstract
The subunit composition of the NAD(P)H dehydrogenase complex of Thermosynechococcus elongatus was analyzed by different types of mass spectrometry. All 15 known subunits (NdhA-NdhO) were identified in the purified NDH-1L complex. Moreover, two additional intact mass tags of 4902.7 and 4710.5 Da could be assigned after reannotation of the T. elongatus genome. NdhP and NdhQ are predicted to contain a single transmembrane helix each, and homologues are apparent in other cyanobacteria. Additionally, ndhP is present in some cyanophages in a cluster of PSI genes and exhibits partial similarity to NDF6, a subunit of the plant NDH-1 complex.
摘要
采用不同类型的质谱分析法分析了嗜热栖热菌 NAD(P)H 脱氢酶复合物的亚基组成。在纯化的 NDH-1L 复合物中鉴定出了所有 15 种已知亚基（NdhA-NdhO）。此外，在重新注释嗜热栖热菌基因组后，还可以分配两个额外的完整质量标签，分别为 4902.7 和 4710.5 Da。预测 NdhP 和 NdhQ 各包含一个单一的跨膜螺旋，在其他蓝细菌中也有明显的同源物。此外，在一些噬藻体的 PSI 基因簇中存在 ndhP，并与植物 NDH-1 复合物的一个亚基 NDF6 具有部分相似性。
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