Unit of Cellular Signaling, Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
J Biol Chem. 2011 Apr 29;286(17):15268-77. doi: 10.1074/jbc.M110.211813. Epub 2011 Jan 18.
We report that the activation level of AMP-dependent protein kinase AMPK is elevated in cancer cell lines as a hallmark of their transformed state. In OVCAR3 and A431 cells, c-Src signals through protein kinase Cα, phospholipase Cγ, and LKB1 to AMPK. AMPK controls internal ribosome entry site (IRES) dependent translation in these cells. We suggest that AMPK activation via PKC might be a general mechanism to regulate IRES-dependent translation in cancer cells.
我们报告称，作为其转化状态的标志，AMP 依赖的蛋白激酶 AMPK 的激活水平在癌细胞系中升高。在 OVCAR3 和 A431 细胞中，c-Src 通过蛋白激酶 Cα、磷脂酶 Cγ 和 LKB1 信号传递至 AMPK。AMPK 控制这些细胞中的内部核糖体进入位点（IRES）依赖的翻译。我们认为，PKC 介导的 AMPK 激活可能是调节癌细胞中 IRES 依赖翻译的一般机制。
