Nagata K, Satoh T, Itoh H, Kozasa T, Okano Y, Doi T, Kaziro Y, Nozawa Y
Department of Biochemistry, Gifu University School of Medicine, Japan.
FEBS Lett. 1990 Nov 26;275(1-2):29-32. doi: 10.1016/0014-5793(90)81431-m.
A novel low Mr GTP-binding protein cDNA was isolated from a rat megakaryocyte cDNA library with a synthetic oligonucleotide probe corresponding to an 8-amino acid sequence specific for c25KG, a GTP-binding protein previously isolated from human platelet cytosol fraction [(1989) J. Biol. Chem. 264, 17000-17005]. The cDNA has an open reading frame encoding a protein of 221 amino acids with a calculated Mr of 25068. The protein is designated as ram (ras-related gene from megakaryocyte) protein (ram p25). The amino acid sequence deduced from the ram cDNA contains the consensus sequences for GTP-binding and GTPase domains. ram p25 shares about 23%, 39% and 80% amino acid homology with the H-ras, smg25A and c25KG proteins, respectively. The 3.5-kb ram mRNA was detected abundantly in spleen cells.
用与c25KG（一种先前从人血小板胞质溶胶组分中分离出的GTP结合蛋白[(1989)《生物化学杂志》264, 17000 - 17005]）特异的8个氨基酸序列对应的合成寡核苷酸探针，从大鼠巨核细胞cDNA文库中分离出一个新的低分子量GTP结合蛋白cDNA。该cDNA有一个编码221个氨基酸的开放阅读框，计算出的分子量为25068。该蛋白被命名为ram（来自巨核细胞的ras相关基因）蛋白（ram p25）。从ram cDNA推导的氨基酸序列含有GTP结合和GTP酶结构域的共有序列。ram p25与H-ras、smg25A和c25KG蛋白分别有大约23%、39%和80%的氨基酸同源性。在脾细胞中大量检测到3.5-kb的ram mRNA。
