Federal University of Santa Maria, Brazil.
J Adhes Dent. 2010 Dec;12(6):435-42. doi: 10.3290/j.jad.a18242.
the aim of this study was to evaluate the effect of dental conditioners in terms of dentin micromorphology and the thickness of the hybrid layer obtained by using the self-etching adhesive systems Adhe SE (AD), G Bond (GB), Adper Prompt L Pop (LP) and Tyrian (TY) and the etch-and-rinse Single Bond (SB).
dentin sticks from 50 third human molars were obtained by first cutting dentin disks out of crowns; the disks were subsequently sectioned into sticks. The sticks were assigned to experimental groups as follows: G1: phosphoric acid 37%, Single Bond (SB); G2: Adhe SE (AD); G3: G Bond (GB); G4: Adper Prompt L-Pop (LP); G5: Tyrian SPE (TY). Half the samples served to investigate the dentin micromorphology. To this end, the proprietary conditioner of the respective adhesive system was applied to these samples, and conditioner effects were examined with SEM. The other half of the samples was hybridized and restored with composite resin to investigate the thickness of the hybrid layer, which was also evaluated with SEM. Statistical analysis was performed with ANOVA, Tukey's, and the Kruskal-Wallis tests.
SEM observation showed significant differences of the hybrid layer thickness. LP presented the highest average thickness, 3.45 microm, while SB presented an average of 2.44 microm and AD an average of 1.43 microm. The averages of TY and SB did not show any statistically significant differences. The adhesive system GB presented the lowest average in hybrid layer, 0.37 microm. The micromorphological evaluation showed that the conditioners commonly removed the smear layer.
all the micromorphological alterations and the hybrid layer thickness show that the adhesive systems studied performed distinct actions. Although there is no apparent correlation of the pH of the conditioners with the thickness of the hybrid layer, it is clear that more acidic conditioners promote more severe transformations in the dentin micromorphology.
本研究旨在评估牙本质调节剂对自酸蚀粘接系统 Adhe SE（AD）、G Bond（GB）、Adper Prompt L Pop（LP）和 Tyrian（TY）以及全酸蚀粘接剂 Single Bond（SB）获得的牙本质微观形态和混合层厚度的影响。
从 50 个人类第三磨牙中获得牙本质棒，首先从牙冠上切出牙本质片，然后将这些牙本质片切成棒状。将这些棒状样本分为以下实验组：G1：磷酸 37%，Single Bond（SB）；G2：Adhe SE（AD）；G3：G Bond（GB）；G4：Adper Prompt L-Pop（LP）；G5：Tyrian SPE（TY）。一半的样本用于研究牙本质微观形态。为此，将各粘接系统的专用调节剂应用于这些样本，并使用 SEM 观察调节剂的效果。另一半样本进行混合和复合树脂修复，以研究混合层厚度，同样使用 SEM 进行评估。采用方差分析、Tukey 检验和 Kruskal-Wallis 检验进行统计学分析。
SEM 观察显示混合层厚度存在显著差异。LP 呈现出最高的平均厚度 3.45μm，而 SB 呈现平均 2.44μm，AD 呈现平均 1.43μm。TY 和 SB 的平均值之间没有显著差异。GB 呈现出最低的混合层平均厚度 0.37μm。微观形态评价表明，调节剂通常可去除玷污层。
所有的微观形态改变和混合层厚度表明所研究的粘接系统表现出不同的作用。虽然调节剂的 pH 值与混合层厚度之间似乎没有明显的相关性，但很明显，更酸性的调节剂会促进牙本质微观形态更严重的变化。
