Chinoin Chemical and Pharmaceutical Works Co. Ltd, Member of sanofi-aventis Group, R&D, Analytical Sciences, Budapest, Hungary.
Magn Reson Chem. 2011 Mar;49(3):106-10. doi: 10.1002/mrc.2717. Epub 2011 Jan 18.
A modified version of CPMG-HSQMBC pulse scheme is presented for the measurement of long-range heteronuclear coupling constants. The method implements adiabatic inversion and refocusing pulses on the heteronucleus. Low-power composite 180° XY-16 CPMG pulse train is applied on both proton and X nuclei during the evolution of long-range couplings to eliminate phase distortions due to co-evolution of homonuclear proton-proton couplings. The pulse sequence yields pure absorption antiphase multiplets allowing precise and direct measurement of the (n)J(XH) coupling constants regardless from the size of the proton-proton couplings. The applicability of the method is demonstrated using strychnine as a model compound. The selective 1D version of the method is also presented.
提出了一种改进的 CPMG-HSQMBC 脉冲方案，用于测量长程异核耦合常数。该方法在异核上实现了绝热反转和聚焦脉冲。在长程耦合的演化过程中，在质子和 X 核上施加低功率复合 180°XY-16 CPMG 脉冲序列，以消除由于同核质子-质子耦合的共演化而导致的相位失真。该脉冲序列产生纯吸收反相多重峰，允许精确和直接测量（n）J（XH）耦合常数，而与质子-质子耦合的大小无关。该方法的适用性通过使用士的宁作为模型化合物进行了证明。还提出了该方法的选择性 1D 版本。
